Auxin　plays　an　important　role　in　cell　differentiation,　apical　dominance,　and　tropism　in　plants.　A　new　method　based　on　dispersive　liquid-liquid　microextraction　(DLLME)　combined　with　high-performance　liquid　chromatography-fluorescence　detection　(HPLC-FLD)　has　been　established　to　detect　auxin.　A　mixture　of　CHCl3　(extraction　solvent)　and　acetone　(disperser　solvent)　was　injected　quickly　into　a　sample　solution　with　desired　salt　concentration　and　pH　value,　and　then　a　cloudy　solution　consisting　of　many　dispersed　fine　droplets　of　CHCl3　was　formed.　After　centrifugation,　the　sedimented　phase　was　withdrawn　and　directly　analyzed　by　HPLC-FLD.　Under　optimal　conditions,　four　auxins　were　baseline　separated　within　3.5　min,　with　the　minimal　limit　of　detection　of　0.02　ng　mL(-1)　and　coefficient　correlations　in　the　range　of　0.9980-0.9995.　This　simple　method　was　successfully　applied　to　real　sample　analysis.　Experimental　results　showed　that　DLLME　was　a　high-performance　and　powerful　preconcentration　method　to　extract　and　enrich　related　plant　auxin.