Pressurized　CEC　(pCEC)　with　UV　detection　was　developed　for　the　simultaneous　analysis　of　endogenetic　and　ectogenic　plant　hormones.　Effects　of　some　crucial　factors　including　mobile　phase,　buffer　concentration,　pH　value　and　applied　voltage　on　pCEC　separation　were　investigated,　respectively.　Under　the　optimum　conditions,　namely　a　packed　capillary　column　(3　mu　m　Hypersil　C-18,　45　cm　total　length　with　100　mu　m　id　x　20　cm　effective　length)　as　the　separation　channel;　ACN:　10　mmol/L　phosphate　(50:50,　v/v,　%)　as　the　mobile　phase,　pH　4.0;　pump　flow　rate　0.035　mL/min;　separation　voltage　-10　kV;　UV　wavelength　222　nm,　a　complete　separation　of　five　hormones　was　accomplished.　The　pCEC　assay　presented　good　linearity　(0.8-100　mu　g/mL),　with　LOD　of　0.2　mu　g/mL　and　R　in　range　of　0.998-0.999.　Meanwhile,　a　lack-of-fit　test　was　performed　to　evaluate　these　linear　models.　Results　showed　that　no　significant　lack-of-fit　was　observed　and　these　models　were　appropriate　(alpha　=　0.05).