Burkholderia　cepacia　ZF-16　screened　from　intestinal　contents　of　eel　was　used　as　an　experimental　strain.　The　medium　components　and　fermentation　conditions　for　lipase　production　of　strain　ZF-16　were　studied　by　single　factor　and　uniform　design　tests.　The　optimum　lipase-producing　conditions　were　obtained　and　as　follow:　corn　starch　0.5%,　peptone　3%,　olive　oil　10.0%　(volume　fraction),　MgSO4·7H2O　0.05%,　K2HPO4　0.1%,　initial　pH　of　medium　6.0-7.0,　fermentation　temperature　30,　liquid　volume　50　mL/250　mL,　inoculum　size　2.0%　(volume　fraction),　inoculum　age　18　h,　fermentation　time　68　h.　Under　these　conditions,　the　lipase　activity　of　B.　cepacia　ZF-16　reached　72.59　U/mL,　471%　higher　than　that　before　optimization　(12.71　U/mL).　©　2017,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.