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Burkholderia cepacia ZF-16 screened from intestinal contents of eel was used as an experimental strain. The medium components and fermentation conditions for lipase production of strain ZF-16 were studied by single factor and uniform design tests. The optimum lipase-producing conditions were obtained and as follow: corn starch 0.5%, peptone 3%, olive oil 10.0% (volume fraction), MgSO4·7H2O 0.05%, K2HPO4 0.1%, initial pH of medium 6.0-7.0, fermentation temperature 30, liquid volume 50 mL/250 mL, inoculum size 2.0% (volume fraction), inoculum age 18 h, fermentation time 68 h. Under these conditions, the lipase activity of B. cepacia ZF-16 reached 72.59 U/mL, 471% higher than that before optimization (12.71 U/mL). © 2017, Editorial Office of Journal of CIFST. All right reserved.
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Journal of Chinese Institute of Food Science and Technology
ISSN: 1009-7848
CN: 11-4528/TS
Year: 2017
Issue: 2
Volume: 17
Page: 69-76
Cited Count:
SCOPUS Cited Count: 1
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2
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