• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Li, Shiqi (Li, Shiqi.) [1] | Zhong, Xiaoying (Zhong, Xiaoying.) [2] | Xu, Yanan (Xu, Yanan.) [3] | Zheng, Yunquan (Zheng, Yunquan.) [4] (Scholars:郑允权) | Shi, Xianai (Shi, Xianai.) [5] (Scholars:石贤爱) | Li, Feng (Li, Feng.) [6] (Scholars:李锋) | Guo, Shaobin (Guo, Shaobin.) [7] (Scholars:郭绍彬) | Yang, Jianmin (Yang, Jianmin.) [8] (Scholars:杨建民)

Indexed by:

EI SCIE

Abstract:

Traditional methods for aflatoxin B1 (AFB1) detection are complex, time-consuming, labor-intensive, and high cost. Moreover, they require sophisticated large-scale instrumentation, which limits their on-site rapid detection. Herein, phycocyanin fluorescent nanospheres based on fluorescence immunochromatographic assay were developed for quantitative detection of AFB1 at parts-per-billion (ppb) levels in foodstuffs. Phycocyanin and antiAFB1 monoclonal antibodies were coupled on the surface of latex nanospheres to amplify the fluorescence signal and improve the sensitivity. The fluorescence intensity was measured by a self-developed smartphone-based reading system. Under the optimal conditions, this approach achieved quantitative point-of-care detection of AFB1 within 25 min. The calibration curve for AFB1 was linear in the range of 0.2-48 ppb, and the limit of detection was 0.16 ppb. The practical applicability of the proposed approach was demonstrated by the determination of AFB1 in naturally contaminated samples, and the results were consistent with HPLC detection.

Keyword:

Aflatoxin B1 Immunoassay On-site determination Phycocyanin Smartphone

Community:

  • [ 1 ] [Li, Shiqi]Fuzhou Univ, Coll Chem, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 2 ] [Zhong, Xiaoying]Fuzhou Univ, Coll Chem, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 3 ] [Xu, Yanan]Fuzhou Univ, Coll Chem, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 4 ] [Zheng, Yunquan]Fuzhou Univ, Coll Chem, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 5 ] [Shi, Xianai]Fuzhou Univ, Coll Biol Sci & Engn, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 6 ] [Li, Feng]Fuzhou Univ, Coll Biol Sci & Engn, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 7 ] [Guo, Shaobin]Fuzhou Univ, Coll Biol Sci & Engn, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 8 ] [Yang, Jianmin]Fuzhou Univ, Coll Biol Sci & Engn, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 9 ] [Zheng, Yunquan]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 10 ] [Shi, Xianai]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 11 ] [Li, Feng]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 12 ] [Guo, Shaobin]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China
  • [ 13 ] [Yang, Jianmin]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • 郑允权 杨建民

    [Zheng, Yunquan]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China;;[Yang, Jianmin]Fuzhou Univ, Fujian Key Lab Med Instrument & Pharmaceut Techno, 2 Xueyuan Rd, Fuzhou 350108, Peoples R China

Show more details

Related Keywords:

Source :

FOOD CHEMISTRY

ISSN: 0308-8146

Year: 2021

Volume: 360

9 . 2 3 1

JCR@2021

8 . 5 0 0

JCR@2023

ESI Discipline: AGRICULTURAL SCIENCES;

ESI HC Threshold:84

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 42

SCOPUS Cited Count: 41

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

Online/Total:106/10011769
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1