Dual-signal　point-of-care　testing　(POCT)　method　relying　on　multicolor　changes　have　demonstrated　a　promising　improvement　in　performance.　In　this　work,　we　have　developed　a　highly　sensitive　and　rapid　POCT　method　for　detecting　aflatoxin　B1　(AFB1),　utilizing　a　dual-signal　readout　mode　based　on　temperature　and　multicolor　changes.　A　synthetic　enzyme　mimics,　Al-Cu-Santa　Barbara　Amorphous　material　(Al-Cu-SBA),　has　been　used　to　enable　the　dual-signal　changes　of　gold　nanobipyramids　(Au　NBPs)　for　the　first　time.　Specifically,　the　aptamer　was　immobilized　on　magnetic　beads,　facilitating　hybridization　with　complementary　strands　functionalized　with　Al-Cu-SBA　and　hence　forming　double-stranded　DNA.　Upon　introducing　AFB1　into　the　system,　it　bound　to　the　aptamer　chain,　resulting　in　the　release　of　Al-Cu-SBA　into　solution.　The　liberated　Al-Cu-SBA　was　subsequently　collected　and　used　to　oxidize　3,　3′,　5,　5′-tetramethylbenzidine　(TMB).　The　oxidized　TMB　was　used　to　etch　Au　NBPs,　inducing　significant　color　changes.　Meanwhile,　by　leveraging　the　photothermal　properties　of　Au　NBPs,　we　observed　a　temperature　difference　generated　upon　exposure　to　infrared　laser　irradiation.　This　method　exhibited　a　linear　range　from　0.5　μg·mL−1　to　100　μg·mL−1,　with　a　detection　limit　of　0.167　μg·mL−1.　The　method＇s　applicability　was　extended　to　successfully　detect　AFB1　in　both　peanut　oil　and　milk　samples.　Given　the　application　of　enzyme　mimics　and　the　dual-signal　readout　integrating　temperature　and　multicolor　changes,　our　study　successfully　overcomes　the　limitations　inherent　in　traditional　biological　enzyme-based　methods　and　the　conventional　single-signal　readout　approach.　Additionally,　it　surpasses　the　limitations　associated　with　dual-signal　readout　systems　that　rely　solely　on　monochromatic　colorimetric　analysis.　©　2024　Elsevier　B.V.