Platelet-rich　plasma　(PRP)　has　become　an　important　regenerative　therapy.　However,　the　preparation　method　of　PRP　has　not　been　standardized,　and　the　optimal　platelet　concentration　for　PRP　used　in　skin　wound　repair　is　unclear,　leading　to　inconsistent　clinical　efficacy　of　PRP.　Therefore,　the　development　of　standardized　preparation　methods　for　PRP　and　the　investigation　of　the　dose-response　relationship　between　PRP　with　different　platelet　concentrations　and　tissue　regeneration　plays　an　important　role　in　the　development　and　clinical　application　of　PRP　technology.　This　study　has　developed　an　integrated　blood　collection　device　from　blood　drawing　to　centrifugation.　Response　surface　methodology　was　employed　to　optimize　the　preparation　conditions,　ultimately　achieving　a　platelet　recovery　rate　as　high　as　95.74%　for　PRP　(with　optimal　parameters:　centrifugation　force　1730x　g,　centrifugation　time　10　min,　and　serum　separation　gel　dosage　1.4　g).　Both　in　vitro　and　in　vivo　experimental　results　indicate　that　PRP　with　a　(2x)　enrichment　ratio　is　the　most　effective　in　promoting　fibroblast　proliferation　and　skin　wound　healing,　with　a　cell　proliferation　rate　of　over　150%　and　a　wound　healing　rate　of　78%　on　day　7.